2,785 research outputs found
Experiment to evaluate feasibility of utilizing Skylab-EREP remote sensing data for tectonic analysis of the Bighorn Mountains region, Wyoming-Montana
There are no author-identified significant results in this report
Mixing with the radiofrequency single-electron transistor
By configuring a radio-frequency single-electron transistor as a mixer, we
demonstrate a unique implementation of this device, that achieves good charge
sensitivity with large bandwidth about a tunable center frequency. In our
implementation we achieve a measurement bandwidth of 16 MHz, with a tunable
center frequency from 0 to 1.2 GHz, demonstrated with the transistor operating
at 300 mK. Ultimately this device is limited in center frequency by the RC time
of the transistor's center island, which for our device is ~ 1.6 GHz, close to
the measured value. The measurement bandwidth is determined by the quality
factor of the readout tank circuit.Comment: Submitted to APL september 200
In-situ measurement of the permittivity of helium using microwave NbN resonators
By measuring the electrical transport properties of superconducting NbN
quarter-wave resonators in direct contact with a helium bath, we have
demonstrated a high-speed and spatially sensitive sensor for the permittivity
of helium. In our implementation a mm sensing volume is
measured with a bandwidth of 300 kHz in the temperature range 1.8 to 8.8 K. The
minimum detectable change of the permittivity of helium is calculated to be
/Hz with a sensitivity of order
/Hz easily achievable. Potential applications
include operation as a fast, localized helium thermometer and as a transducer
in superfluid hydrodynamic experiments.Comment: 4 pages, 3 figure
Analysis of the 23 June 2001 M w = 8.4 Peru underthrusting earthquake and its aftershocks
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95298/1/grl16079.pd
Interaction of intermediate energy protons with light nuclei
Interaction of intermediate energy protons with light nucle
A versatile and compact capacitive dilatometer
We describe the design, construction, calibration, and operation of a
relatively simple differential capacitive dilatometer suitable for measurements
of thermal expansion and magnetostriction from 300 K to below 1 K with a
low-temperature resolution of about 0.05 angstroms. The design is characterized
by an open architecture permitting measurements on small samples with a variety
of shapes. Dilatometers of this design have operated successfully with a
commercial physical property measurement system, with several types of
cryogenic refrigeration systems, in vacuum, in helium exchange gas, and while
immersed in liquid helium (magnetostriction only) to temperatures of 30 mK and
in magnetic fields to 45 T.Comment: 8 pages, incorporating 6 figures, submitted to Rev. Sci. Instru
Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes
Heme a is an essential cofactor for function of cytochrome c oxidase in the mitochondrial electron transport chain. Several evolutionarily conserved enzymes have been implicated in the biosynthesis of heme a, including the heme a synthase Cox15. However, the structure of Cox15 is unknown, its enzymatic mechanism and the role of active site residues remain debated, and recent discoveries suggest additional chaperone-like roles for this enzyme. Here, we investigated Cox15 in the model eukaryote Saccharomyces cerevisiae via several approaches to examine its oligomeric states and determine the effects of active site and human pathogenic mutations. Our results indicate that Cox15 exhibits homotypic interactions, forming highly stable complexes dependent upon hydrophobic interactions. This multimerization is evolutionarily conserved and independent of heme levels and heme a synthase catalytic activity. Four conserved histidine residues are demonstrated to be critical for eukaryotic heme a synthase activity and cannot be substituted with other heme-ligating amino acids. The 20-residue linker region connecting the two conserved domains of Cox15 is also important; removal of this linker impairs both Cox15 multimerization and enzymatic activity. Mutations of COX15 causing single amino acid conversions associated with fatal infantile hypertrophic cardiomyopathy and the neurological disorder Leigh syndrome result in impaired stability (S344P) or catalytic function (R217W), and the latter mutation affects oligomeric properties of the enzyme. Structural modeling of Cox15 suggests these two mutations affect protein folding and heme binding, respectively. We conclude that Cox15 multimerization is important for heme a biosynthesis and/or transfer to maturing cytochrome c oxidase
Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes
Heme a is an essential cofactor for function of cytochrome c oxidase in the mitochondrial electron transport chain. Several evolutionarily conserved enzymes have been implicated in the biosynthesis of heme a, including the heme a synthase Cox15. However, the structure of Cox15 is unknown, its enzymatic mechanism and the role of active site residues remain debated, and recent discoveries suggest additional chaperone-like roles for this enzyme. Here, we investigated Cox15 in the model eukaryote Saccharomyces cerevisiae via several approaches to examine its oligomeric states and determine the effects of active site and human pathogenic mutations. Our results indicate that Cox15 exhibits homotypic interactions, forming highly stable complexes dependent upon hydrophobic interactions. This multimerization is evolutionarily conserved and independent of heme levels and heme a synthase catalytic activity. Four conserved histidine residues are demonstrated to be critical for eukaryotic heme a synthase activity and cannot be substituted with other heme-ligating amino acids. The 20-residue linker region connecting the two conserved domains of Cox15 is also important; removal of this linker impairs both Cox15 multimerization and enzymatic activity. Mutations of COX15 causing single amino acid conversions associated with fatal infantile hypertrophic cardiomyopathy and the neurological disorder Leigh syndrome result in impaired stability (S344P) or catalytic function (R217W), and the latter mutation affects oligomeric properties of the enzyme. Structural modeling of Cox15 suggests these two mutations affect protein folding and heme binding, respectively. We conclude that Cox15 multimerization is important for heme a biosynthesis and/or transfer to maturing cytochrome c oxidase
Electronics and data acquisition demonstrator for a kinetic inductance camera
A prototype of digital frequency multiplexing electronics allowing the real
time monitoring of kinetic inductance detector (KIDs) arrays for mm-wave
astronomy has been developed. It requires only 2 coaxial cables for
instrumenting a large array. For that, an excitation comb of frequencies is
generated and fed through the detector. The direct frequency synthesis and the
data acquisition relies heavily on a large FPGA using parallelized and
pipelined processing. The prototype can instrument 128 resonators (pixels) over
a bandwidth of 125 MHz. This paper describes the technical solution chosen, the
algorithm used and the results obtained
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